FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005714956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005714956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2923798
Sequences flagged as poor quality0
Sequence length10-85
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC137780.4712363850033416Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC74970.25641306273552417TruSeq Adapter, Index 16 (100% over 38bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC74580.25507918125670787TruSeq Adapter, Index 16 (100% over 39bp)
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG65760.22491293858194034TruSeq Adapter, Index 7 (97% over 36bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT56060.19173691205753612TruSeq Adapter, Index 16 (100% over 37bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC50410.17241273165930068Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCG42130.14409340180135563TruSeq Adapter, Index 16 (97% over 39bp)
GAGGCTTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC29810.10195642790644223TruSeq Adapter, Index 5 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)