FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005714958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005714958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882179
Sequences flagged as poor quality0
Sequence length10-85
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC145530.5049304710082198Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC76860.266673235770575TruSeq Adapter, Index 16 (100% over 39bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC76850.2666385397992283TruSeq Adapter, Index 16 (100% over 38bp)
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG68700.23836132315168487TruSeq Adapter, Index 7 (97% over 36bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT59320.20581650202849996TruSeq Adapter, Index 16 (100% over 37bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC51510.17871894840674366Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCG42750.14832527750705282TruSeq Adapter, Index 16 (97% over 39bp)
GAGGCTTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC30670.10641254412026456TruSeq Adapter, Index 5 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)