FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3494399
Sequences flagged as poor quality0
Sequence length10-85
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC122060.34930183988720237Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG62270.17819945575762813TruSeq Adapter, Index 7 (100% over 36bp)
GGATGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA48120.13770608336369144TruSeq Adapter, Index 7 (100% over 37bp)
GGATGGCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA36670.10493936153255538TruSeq Adapter, Index 7 (100% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)