FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3837719
Sequences flagged as poor quality0
Sequence length10-85
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGCTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC150060.3910135161016218Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT68560.17864778531205644TruSeq Adapter, Index 7 (100% over 38bp)
CATGGCTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA65510.1707003561229991TruSeq Adapter, Index 7 (100% over 37bp)
CATGGCTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC57850.15074058314326816Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG56830.14808275436528834TruSeq Adapter, Index 7 (100% over 36bp)
CATGGCTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA53320.13893669651165183TruSeq Adapter, Index 7 (100% over 35bp)
CATGGCTGGCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC40910.10659977971289718Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)