FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2950232
Sequences flagged as poor quality0
Sequence length10-85
%GC58

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT63140.21401706713234753TruSeq Adapter, Index 16 (100% over 37bp)
TAGCCTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG49530.16788510191740855TruSeq Adapter, Index 7 (97% over 36bp)
TAGCCTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC36160.12256663204792029Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGCCTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC35870.12158365850550058TruSeq Adapter, Index 16 (100% over 38bp)
TAGCCTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC32500.11016082802979563TruSeq Adapter, Index 16 (100% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)