FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4751966
Sequences flagged as poor quality0
Sequence length10-85
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC199960.42079425652456265TruSeq Adapter, Index 7 (100% over 42bp)
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG173830.36580648935619486TruSeq Adapter, Index 7 (100% over 36bp)
GACGACCAGATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA120350.2532635965829722TruSeq Adapter, Index 7 (100% over 35bp)
GACGACCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA80220.16881433915983407TruSeq Adapter, Index 7 (100% over 37bp)
GACGACCAGATTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC70360.14806503245183153Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)