FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247950
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT15720.6339987900786449TruSeq Adapter, Index 2 (100% over 37bp)
CTGGTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT9740.39282113329300267TruSeq Adapter, Index 2 (100% over 39bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC9360.3774954627949183Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA7760.31296632385561607TruSeq Adapter, Index 2 (100% over 36bp)
CTGGTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG6210.250453720508167TruSeq Adapter, Index 2 (100% over 38bp)
CTGGTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC4920.19842710223835452Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA4570.18431135309538213TruSeq Adapter, Index 2 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)