FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244743
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT15490.6329088063805707TruSeq Adapter, Index 2 (100% over 37bp)
CTGGTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT9430.38530213325815243TruSeq Adapter, Index 2 (100% over 39bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC8440.344851538144094Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA7260.29663769750309504TruSeq Adapter, Index 2 (100% over 36bp)
CTGGTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG5930.24229497881451154TruSeq Adapter, Index 2 (100% over 38bp)
CTGGTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC4920.20102719996077517Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA4640.18958662760528391TruSeq Adapter, Index 2 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)