FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249942
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT15790.6317465652031271TruSeq Adapter, Index 2 (100% over 37bp)
CTGGTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT9090.3636843747749478TruSeq Adapter, Index 2 (100% over 39bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC8780.3512814973073753Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA7550.30207008025862003TruSeq Adapter, Index 2 (100% over 36bp)
CTGGTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG6090.243656528314569TruSeq Adapter, Index 2 (100% over 38bp)
CTGGTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC5230.20924854566259374Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA4650.18604316201358714TruSeq Adapter, Index 2 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)