FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005715192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509537
Sequences flagged as poor quality0
Sequence length10-85
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC37610.7381210785477796TruSeq Adapter, Index 4 (100% over 37bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC19370.38014903726324095Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA19310.3789714976537523TruSeq Adapter, Index 2 (97% over 36bp)
CTGGTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA19240.3775977014426823TruSeq Adapter, Index 4 (100% over 39bp)
CTGGTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC13710.26906780076814835TruSeq Adapter, Index 4 (100% over 38bp)
CTGGTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT12470.2447319821720503Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA8890.17447211880589633TruSeq Adapter, Index 4 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)