FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005715194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501380
Sequences flagged as poor quality0
Sequence length10-85
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC35490.7078463440903108TruSeq Adapter, Index 4 (100% over 37bp)
CTGGTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA19070.3803502333559376TruSeq Adapter, Index 4 (100% over 39bp)
CTGGTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA18850.3759623439307511TruSeq Adapter, Index 2 (97% over 36bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC18350.3659898679644182Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC13340.26606565878176236TruSeq Adapter, Index 4 (100% over 38bp)
CTGGTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT12310.24552235829111654Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA8240.16434640392516653TruSeq Adapter, Index 4 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)