FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509119
Sequences flagged as poor quality0
Sequence length10-85
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC36210.7112286125640567TruSeq Adapter, Index 4 (100% over 37bp)
CTGGTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA19210.37731846582036815TruSeq Adapter, Index 2 (97% over 36bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC18910.37142593381900896Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA18390.36121221168331963TruSeq Adapter, Index 4 (100% over 39bp)
CTGGTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC13210.2594678257931839TruSeq Adapter, Index 4 (100% over 38bp)
CTGGTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT12680.24905768592411595Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA9030.17736521324091226TruSeq Adapter, Index 4 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)