FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246888
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA5310.21507728200641585TruSeq Adapter, Index 2 (97% over 36bp)
TCCATTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5130.20778652668416447Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC4020.16282686886361428TruSeq Adapter, Index 4 (100% over 37bp)
TCCATTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA3200.12961342795113573TruSeq Adapter, Index 4 (100% over 40bp)
TCCATTGGTTTTACCAATGGAGATCGGAAGAGCACACGTCTGAACTCCAG2580.10450082628560318Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCATTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2500.10126049058682479Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)