FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129759
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAA1810.1394893610462473No Hit
TAATAGGATTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1690.13024144760671708Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAAAAAAAAAAA1630.12561749088695195No Hit
AAAAAAAAAAAAAAA1620.12484683143365778No Hit
AAAAAAAAAAAAAA1570.12099353416718686No Hit
AAAAAAAAAAAAAAAAAA1390.10712166400789155No Hit
AAAAAAAAAAAA1310.10095638838153809No Hit
AAAAAAAAAAAAAAAAA1300.1001857289282439No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)