FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131574
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATAGGATTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2090.15884597260857009Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAAAAAAAAAAAA1970.14972562968367611No Hit
AAAAAAAAAAAAAAA1770.13452505814218615No Hit
AAAAAAAAAAAAAAAA1720.13072491525681365No Hit
AAAAAAAAAAAAA1600.12160457233191968No Hit
AAAAAAAAAAAAAAAAA1500.1140042865611747No Hit
AAAAAAAAAAAAAAAAAA1430.1086840865216532No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)