FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210766
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATAGGATTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4520.21445584202385584Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAATAGGATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2500.11861495687160169Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAATAGGATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC2300.10912576032187354TruSeq Adapter, Index 4 (100% over 37bp)
AAAAAAAAAAAAAA2170.10295778256455025No Hit
TAATAGGATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA2160.10248332273706386TruSeq Adapter, Index 2 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)