FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216065
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATAGGATTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5320.24622220165228056Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAATAGGATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2770.12820216138661977Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAAAAAAAAAAAAAA2270.10506097702080391No Hit
TAATAGGATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC2240.10367250595885497TruSeq Adapter, Index 4 (100% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)