FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713337
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC40930.5737820973817425Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA20880.2927087757960123TruSeq Adapter, Index 2 (100% over 36bp)
GGATGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT18110.2538771996966371TruSeq Adapter, Index 2 (100% over 37bp)
GGATGGCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC13450.18855043268469182Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT11020.15448518722567314TruSeq Adapter, Index 2 (100% over 39bp)
GGATGGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA10330.1448123397496555TruSeq Adapter, Index 2 (100% over 40bp)
GGATGGCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG9280.13009278924267212TruSeq Adapter, Index 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph