FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720670
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC40060.5558716194652199Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA21490.2981947354545076TruSeq Adapter, Index 2 (100% over 36bp)
GGATGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT18230.252959051993284TruSeq Adapter, Index 2 (100% over 37bp)
GGATGGCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC12700.1762249018274661Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT10770.14944426714029999TruSeq Adapter, Index 2 (100% over 39bp)
GGATGGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA10660.14791790972289676TruSeq Adapter, Index 2 (100% over 40bp)
GGATGGCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG8620.11961091761832739TruSeq Adapter, Index 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)