FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715106
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT40440.5655105676640946Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA20780.29058629070375586TruSeq Adapter, Index 2 (97% over 36bp)
GGATGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC17390.24318073124823453TruSeq Adapter, Index 4 (100% over 37bp)
GGATGGCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC12830.17941396100717935Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA9130.1276733798905337TruSeq Adapter, Index 4 (100% over 39bp)
GGATGGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA9000.12585546758102995TruSeq Adapter, Index 4 (100% over 40bp)
GGATGGCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG8960.12529610994733648TruSeq Adapter, Index 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)