FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700848
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTAGTGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC14120.20147021893477615TruSeq Adapter, Index 4 (100% over 37bp)
GAGCTAGTGATTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT11100.15837956304362716Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGCTAGTGATTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7880.11243522133187224Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGCTAGTGATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG7740.11043764125744811TruSeq Adapter, Index 2 (97% over 35bp)
GAGCTAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT7070.10087779375841836TruSeq Adapter, Index 4 (100% over 41bp)
GAGCTAGTGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA7050.10059242517635779TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)