FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195455
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACCTCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC25301.2944155943823386Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCTCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG6060.3100457905911847TruSeq Adapter, Index 2 (100% over 35bp)
TAACCTCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT3460.17702284413292063TruSeq Adapter, Index 2 (100% over 39bp)
TAACCTCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3460.17702284413292063Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCTCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG2440.12483691898390935TruSeq Adapter, Index 2 (100% over 38bp)
TAACCTCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT2240.11460438464096596TruSeq Adapter, Index 2 (100% over 37bp)
TAACCTCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA2150.10999974418664142TruSeq Adapter, Index 2 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)