FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005715267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193464
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACCTCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC25211.3030848116445437Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCTCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG5980.3091014348922797TruSeq Adapter, Index 2 (100% over 35bp)
TAACCTCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3670.18969937559442585Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCTCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT3500.18091221105735433TruSeq Adapter, Index 2 (100% over 39bp)
TAACCTCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG2470.12767233180333293TruSeq Adapter, Index 2 (100% over 38bp)
TAACCTCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA2220.11475003101352191TruSeq Adapter, Index 2 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)