FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197499
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACCTCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC26111.3220320102886598Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCTCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG5720.2896217196036436TruSeq Adapter, Index 2 (100% over 35bp)
TAACCTCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3820.19341870085418156Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCTCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT3610.18278573562397785TruSeq Adapter, Index 2 (100% over 39bp)
TAACCTCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA2480.12557025605192937TruSeq Adapter, Index 2 (100% over 40bp)
TAACCTCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG2370.12000060759801315TruSeq Adapter, Index 2 (100% over 38bp)
TAACCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT2100.10632965230203696TruSeq Adapter, Index 2 (100% over 41bp)
TAACCTCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1990.10076000384812075TruSeq Adapter, Index 2 (100% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)