FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194798
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACCTCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC26001.334715962176203Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCTCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG5980.3069846713005267TruSeq Adapter, Index 2 (100% over 35bp)
TAACCTCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT3490.179159950307498TruSeq Adapter, Index 2 (100% over 39bp)
TAACCTCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3310.16991960903089354Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCTCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG2390.12269119806158175TruSeq Adapter, Index 2 (100% over 38bp)
TAACCTCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA2370.12166449347529235TruSeq Adapter, Index 2 (100% over 40bp)
TAACCTCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT2130.10934403843981971TruSeq Adapter, Index 2 (100% over 37bp)
TAACCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT2090.10729062926724094TruSeq Adapter, Index 2 (100% over 41bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)