FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208209
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACCTCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT35401.7002146881258735Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCTCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG8040.3861504545913001TruSeq Adapter, Index 2 (97% over 35bp)
TAACCTCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4680.22477414520986125Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT4240.20364153326705375TruSeq Adapter, Index 4 (100% over 43bp)
TAACCTCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA4010.19259494066058624TruSeq Adapter, Index 4 (100% over 39bp)
TAACCTCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC3380.16233688265156646TruSeq Adapter, Index 4 (100% over 38bp)
CTGGTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT3200.15369172322041796TruSeq Adapter, Index 4 (100% over 41bp)
TAACCTCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA2810.13496054445292952TruSeq Adapter, Index 4 (100% over 40bp)
TAACCTCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC2600.12487452511658959TruSeq Adapter, Index 4 (100% over 37bp)
TAACCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT2330.11190678596986682TruSeq Adapter, Index 4 (100% over 41bp)
TAACCTCCTGTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2250.10806449288935636Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)