FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204741
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACCTCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT33851.6533083261291093Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCTCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG7880.3848765025080467TruSeq Adapter, Index 2 (97% over 35bp)
TAACCTCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA4380.2139288173839143TruSeq Adapter, Index 4 (100% over 39bp)
CTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT4260.20806775389394402TruSeq Adapter, Index 4 (100% over 43bp)
TAACCTCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4080.19927615865898868Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT3430.1675287314216498TruSeq Adapter, Index 4 (100% over 41bp)
TAACCTCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC3050.14896869703674398TruSeq Adapter, Index 4 (100% over 38bp)
TAACCTCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC2610.12747813090685306TruSeq Adapter, Index 4 (100% over 37bp)
TAACCTCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA2600.12698970894935552TruSeq Adapter, Index 4 (100% over 40bp)
TAACCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT2330.11380231609692244TruSeq Adapter, Index 4 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)