FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207306
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACCTCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT34481.6632417778549582Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCTCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG7840.3781849054055358TruSeq Adapter, Index 2 (97% over 35bp)
CTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT4880.23540080846671105TruSeq Adapter, Index 4 (100% over 43bp)
TAACCTCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4670.22527085564334848Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCTCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA4080.1968105119967584TruSeq Adapter, Index 4 (100% over 39bp)
TAACCTCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC3240.15629070070330814TruSeq Adapter, Index 4 (100% over 37bp)
CTGGTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT3150.15194929235043847TruSeq Adapter, Index 4 (100% over 41bp)
TAACCTCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC2950.14230171823295032TruSeq Adapter, Index 4 (100% over 38bp)
TAACCTCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA2920.1408545821153271TruSeq Adapter, Index 4 (100% over 40bp)
TAACCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT2580.12445370611559724TruSeq Adapter, Index 4 (100% over 41bp)
TAACCTCCTGTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2240.10805283011586736Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCTCCTGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA2160.10419380046887211TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)