FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173117
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTAGGAGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7680.44363060820138983Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGGAGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC6490.3748909696910182Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGGAGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT4490.25936216547190627TruSeq Adapter, Index 2 (100% over 37bp)
TTGGTAGGAGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA2690.15538624167470555TruSeq Adapter, Index 2 (100% over 36bp)
TTGGTAGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG2410.13921220908402987TruSeq Adapter, Index 2 (100% over 38bp)
TTGGTAGGAGTTACCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2250.1299699047465009Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGTAGGAGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG1910.11033000802925189TruSeq Adapter, Index 2 (100% over 35bp)
TTGGTAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA1750.10108770369172293TruSeq Adapter, Index 2 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)