FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173767
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTAGGAGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC8230.47362272468305255Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGGAGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC6410.36888477098643585Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGGAGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT4360.25091070226222467TruSeq Adapter, Index 2 (100% over 37bp)
TTGGTAGGAGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA2580.14847468161388525TruSeq Adapter, Index 2 (100% over 36bp)
TTGGTAGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG2430.1398424326828454TruSeq Adapter, Index 2 (100% over 38bp)
TTGGTAGGAGTTACCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1850.10646440348282471Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)