FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172375
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTAGGAGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7650.4437998549673677Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGGAGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC6500.3770848440899202Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGGAGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT4510.2616388687454677TruSeq Adapter, Index 2 (100% over 37bp)
TTGGTAGGAGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA2780.16127628716461204TruSeq Adapter, Index 2 (100% over 36bp)
TTGGTAGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG2430.14097171863669325TruSeq Adapter, Index 2 (100% over 38bp)
TTGGTAGGAGTTACCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1760.10210297316896302Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)