FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243322
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTAGGAGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC11800.48495409375231174Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGGAGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT10070.41385489187167623Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGGAGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC7240.297548104980232TruSeq Adapter, Index 4 (100% over 37bp)
TTGGTAGGAGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA4280.17589860349660122TruSeq Adapter, Index 2 (97% over 36bp)
TTGGTAGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC3550.14589720617124632TruSeq Adapter, Index 4 (100% over 38bp)
TTGGTAGGAGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG3030.12452634780250038TruSeq Adapter, Index 2 (97% over 35bp)
TTGGTAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA2940.12082754539252513TruSeq Adapter, Index 4 (100% over 40bp)
TTGGTAGGAGTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2640.10849820402594093Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)