FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237641
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTAGGAGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC11830.49780972138646107Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGGAGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT10190.42879806094066264Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGGAGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC6810.2865667119731023TruSeq Adapter, Index 4 (100% over 37bp)
TTGGTAGGAGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA4000.16832112303853292TruSeq Adapter, Index 2 (97% over 36bp)
TTGGTAGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC3480.14643937704352364TruSeq Adapter, Index 4 (100% over 38bp)
TTGGTAGGAGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG3190.13423609562323TruSeq Adapter, Index 2 (97% over 35bp)
TTGGTAGGAGTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2580.10856712435985373Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA2550.10730471593706473TruSeq Adapter, Index 4 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)