FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244875
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTAGGAGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC12490.5100561510974987Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGGAGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT10830.4422664624808576Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGGAGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC7050.2879019908116386TruSeq Adapter, Index 4 (100% over 37bp)
TTGGTAGGAGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA4030.16457376212353242TruSeq Adapter, Index 2 (97% over 36bp)
TTGGTAGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC3330.13598774885145481TruSeq Adapter, Index 4 (100% over 38bp)
TTGGTAGGAGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG2830.11556916794282798TruSeq Adapter, Index 2 (97% over 35bp)
TTGGTAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA2630.10740173557937722TruSeq Adapter, Index 4 (100% over 40bp)
TTGGTAGGAGTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2480.10127616130678918Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)