FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005715303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005715303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397103
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAACTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC6350.15990813466531353Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4710.11860902586986247Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)