FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721543
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCTTGACTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT38950.5398153678990719Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG18730.25958258897945097TruSeq Adapter, Index 3 (100% over 38bp)
TTACCTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC17780.24641636049410778TruSeq Adapter, Index 3 (100% over 39bp)
TTACCTTGACTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC17100.23699211273617787Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG12400.17185392970342725TruSeq Adapter, Index 3 (100% over 37bp)
TTACCTTGACTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA7870.10907180861015905TruSeq Adapter, Index 3 (100% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)