FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704955
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCTTGACTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT37790.5360625855551064Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG18030.25576100602166096TruSeq Adapter, Index 3 (100% over 38bp)
TTACCTTGACTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC17570.24923576682199575Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC17210.24412905788312728TruSeq Adapter, Index 3 (100% over 39bp)
TTACCTTGACTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG12070.17121660247817239TruSeq Adapter, Index 3 (100% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)