FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739422
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCTTGACTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT39420.5331191119550135Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG18830.25465836829307215TruSeq Adapter, Index 3 (100% over 38bp)
TTACCTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC17920.24235145830121368TruSeq Adapter, Index 3 (100% over 39bp)
TTACCTTGACTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC17820.24099905060980062Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG11860.1603955522015845TruSeq Adapter, Index 3 (100% over 37bp)
TTACCTTGACTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA7870.10643448531420488TruSeq Adapter, Index 3 (100% over 36bp)
TTACCTTGACTTACCAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7470.10102485454855278Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)