FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611280
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA32030.5239824630283993TruSeq Adapter, Index 3 (100% over 36bp)
GACGACCAGATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT21510.35188457008244994TruSeq Adapter, Index 1 (97% over 35bp)
GACGACCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG16000.261745844784714TruSeq Adapter, Index 3 (100% over 37bp)
GACGACCAGATTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT13080.21397722811150374Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC8290.13561706582907995TruSeq Adapter, Index 3 (100% over 42bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)