FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628438
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA32690.5201786015486014TruSeq Adapter, Index 3 (100% over 36bp)
GACGACCAGATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT21910.348642189046493TruSeq Adapter, Index 1 (97% over 35bp)
GACGACCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG15720.2501440078416646TruSeq Adapter, Index 3 (100% over 37bp)
GACGACCAGATTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT13630.21688694827492927Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC8330.13255086420617468TruSeq Adapter, Index 3 (100% over 42bp)
GACGACCAGATTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6480.10311279712557167Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)