FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538101
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT53700.9979539157147079Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA41300.7675139053820751TruSeq Adapter, Index 3 (100% over 36bp)
GCTTGCCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG32770.608993478919385TruSeq Adapter, Index 3 (100% over 37bp)
GCTTGCCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT20710.3848719849990987TruSeq Adapter, Index 1 (97% over 35bp)
GCTTGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC15840.2943685293281373TruSeq Adapter, Index 3 (100% over 39bp)
CATTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC14270.26519185060053785TruSeq Adapter, Index 3 (100% over 42bp)
GCTTGCCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG11310.21018359006952225TruSeq Adapter, Index 3 (100% over 38bp)
GCTTGCCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA6270.11652087619238767TruSeq Adapter, Index 3 (100% over 40bp)
GCTTGCCATTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5720.10629974670182735Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT5720.10629974670182735TruSeq Adapter, Index 3 (100% over 43bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)