FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526124
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT52250.9931118899727062Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA41020.7796641096015389TruSeq Adapter, Index 3 (100% over 36bp)
GCTTGCCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG30640.5823722164356692TruSeq Adapter, Index 3 (100% over 37bp)
GCTTGCCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT20040.380898799522546TruSeq Adapter, Index 1 (97% over 35bp)
GCTTGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC15480.2942272163976553TruSeq Adapter, Index 3 (100% over 39bp)
CATTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC14530.27617063657996976TruSeq Adapter, Index 3 (100% over 42bp)
GCTTGCCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG10800.20527480213789906TruSeq Adapter, Index 3 (100% over 38bp)
GCTTGCCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA6380.12126418867035149TruSeq Adapter, Index 3 (100% over 40bp)
GCTTGCCATTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5720.10871961742859099Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT5390.10244733180771073TruSeq Adapter, Index 3 (100% over 43bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)