FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552652
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT55311.0008106367117102Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA43030.7786093237697502TruSeq Adapter, Index 3 (100% over 36bp)
GCTTGCCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG32240.5833689193199337TruSeq Adapter, Index 3 (100% over 37bp)
GCTTGCCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT20450.3700339454123029TruSeq Adapter, Index 1 (97% over 35bp)
GCTTGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC17190.31104564897982817TruSeq Adapter, Index 3 (100% over 39bp)
CATTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC14250.2578476147738541TruSeq Adapter, Index 3 (100% over 42bp)
GCTTGCCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG11370.20573525473534882TruSeq Adapter, Index 3 (100% over 38bp)
GCTTGCCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA6550.1185194299486838TruSeq Adapter, Index 3 (100% over 40bp)
GCTTGCCATTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5850.10585323132821377Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)