FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005716281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535004
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT54251.0140111101973068Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA42810.8001809332266675TruSeq Adapter, Index 3 (100% over 36bp)
GCTTGCCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG32520.6078459226473073TruSeq Adapter, Index 3 (100% over 37bp)
GCTTGCCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT20290.3792495009383107TruSeq Adapter, Index 1 (97% over 35bp)
GCTTGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC16090.3007454149875515TruSeq Adapter, Index 3 (100% over 39bp)
CATTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC15070.2816801369709385TruSeq Adapter, Index 3 (100% over 42bp)
GCTTGCCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG10350.19345649752151386TruSeq Adapter, Index 3 (100% over 38bp)
GCTTGCCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA6720.1256065375212148TruSeq Adapter, Index 3 (100% over 40bp)
CATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT5560.10392445663957653TruSeq Adapter, Index 3 (100% over 43bp)
GCTTGCCATTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5550.10373754214921757Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)