FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481560
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTACTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC25730.5343051748484093Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTACTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG8910.18502367306254672TruSeq Adapter, Index 3 (100% over 37bp)
CATTACTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT8430.17505606777971594Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTACTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT6870.14266135061051582TruSeq Adapter, Index 1 (97% over 35bp)
CATTACTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA5940.12334911537503115TruSeq Adapter, Index 3 (100% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)