FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478580
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTACTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC24870.5196623344059509Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTACTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG8740.18262359480128715TruSeq Adapter, Index 3 (100% over 37bp)
CATTACTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT8710.18199674035688912Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTACTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT7360.15378829035897865TruSeq Adapter, Index 1 (97% over 35bp)
CATTACTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA5340.11158009110284592TruSeq Adapter, Index 3 (100% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)