FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513118
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA66561.297167513125636TruSeq Adapter, Index 3 (100% over 36bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT41190.8027393309141367Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC38940.7588897680455567Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG36450.710362918470995TruSeq Adapter, Index 3 (100% over 38bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG36230.7060754056571783TruSeq Adapter, Index 3 (100% over 37bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC35990.7013981189511964TruSeq Adapter, Index 3 (100% over 39bp)
GAGGCTTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT24500.47747301790231483TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC14460.28180652403540707Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA13400.26114850775065385TruSeq Adapter, Index 3 (100% over 36bp)
GAGGCTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA12670.2469217606866257TruSeq Adapter, Index 3 (100% over 40bp)
GAGGCTTGGTTTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT12550.24458311733363478TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT10120.19722558943556842TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT9890.19274318967566914Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7190.14012371423337322Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT6870.13388733195873073TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTACCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6090.1186861501642897Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)