FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534830
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA67921.269936241422508TruSeq Adapter, Index 3 (100% over 36bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT43480.8129686068470355Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC40290.7533234859675036Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG37990.7103191668380607TruSeq Adapter, Index 3 (100% over 38bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC36670.6856384271637717TruSeq Adapter, Index 3 (100% over 39bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG36490.6822728717536414TruSeq Adapter, Index 3 (100% over 37bp)
GAGGCTTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT24650.46089411588729134TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC14970.2799020249425051Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA14520.2714881364171793TruSeq Adapter, Index 3 (100% over 36bp)
GAGGCTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA13010.24325486603219718TruSeq Adapter, Index 3 (100% over 40bp)
GAGGCTTGGTTTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT12470.2331581998018062TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT9880.1847315969560421Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT9830.18379672045322815TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7710.14415795673391546Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT7450.13929659891928275TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTACCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6060.11330703214105417Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)