FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521704
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA68061.3045711744590802TruSeq Adapter, Index 3 (100% over 36bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT41270.7910615981476086Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC40460.775535552727217Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG38320.7345161240856884TruSeq Adapter, Index 3 (100% over 38bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC37000.7092144204376428TruSeq Adapter, Index 3 (100% over 39bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG36440.6984803643445325TruSeq Adapter, Index 3 (100% over 37bp)
GAGGCTTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT24400.4676981583426617TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC15180.2909695919525248Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA13800.2645178108659317TruSeq Adapter, Index 3 (100% over 36bp)
GAGGCTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA13450.2578090258077377TruSeq Adapter, Index 3 (100% over 40bp)
GAGGCTTGGTTTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT12850.24630825142226243TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT9920.19014613650652476TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT9760.1870792633370647Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7410.14203456366061984Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT6720.12880867311732325TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTACCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6190.11864965574348674Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)