FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282182
Sequences flagged as poor quality0
Sequence length10-85
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA10810.3830860933723625TruSeq Adapter, Index 2 (97% over 36bp)
GCTTGCCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC10170.36040569561488683TruSeq Adapter, Index 4 (100% over 37bp)
GCTTGCCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT9370.33205519841804226Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC7310.2590526681361674TruSeq Adapter, Index 4 (100% over 42bp)
GCTTGCCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG4950.1754187014054759TruSeq Adapter, Index 2 (97% over 35bp)
GCTTGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA4110.14565067934878909TruSeq Adapter, Index 4 (100% over 39bp)
GCTTGCCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC3540.1254509500960373TruSeq Adapter, Index 4 (100% over 38bp)
CATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT3320.11765456336690504TruSeq Adapter, Index 4 (100% over 43bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)